CONSERT Labcourse, Lentiviral vectors: concepts, practice, hope and reality 

Course tailored to the EC-funded CONSERT project (www.genetherapy.nl)

Programme

June 29^th  14h00:  Registration of participants, posters and exhibits set up 

15h00 - 16h00:  Welcome addresses:
Anne GALY (Généthon/INSERM, Evry, F), Stefan CONSTANTINESCU (Ludwig Institute/EASCO, Brussels, B)

16h00 - 18h30: Opening Session     Chair: Gerard WAGEMAKER
Gerard Wagemaker (Erasmus University, Rotterdam, NL): CONSERT project and hematopoietic stem cell biology (40 min)
16h40 - 17h10: coffee break
Olivier DANOS (Necker Hospital, Paris, F) From retroviruses to therapeutic tools  (40 min) 
Mary COLLINS (UCL, London, UK) General and advanced applications of lentiviral vectors (40 min)

18h30: Welcome cocktail

June 30^th

9h00 - 12h40:  Session 1: Biology and vectorology   Chair: Frederic BUSHMAN
Gregory TOWERS (UCL, London, UK) TRIMs and Cyclophilins: restriction factors influencing viral tropism (40 min)
Pierre CHARNEAU (Pasteur Institute, Paris, F)   The roles of HIV flap  (40 min)
Els Verhoeyen (ENS-INSERM, Lyon, F)   Envelope pseudotypes for lentiviral vectors (40 min)
11h00 - 11h30: coffee break 
11h30 - 12h10: Axel Schambach (Hannover Medical School, D)   Vector design, concepts and novelties (40 min)
12h10 - 12h40: Selected communications (15 min)
Minna KAIKKONEN (ARK Therapeutics, Kuopio, FI) Avidin-displaying Lentiviruses as Versatile Tools for Targeting and Dual-imaging of Gene Delivery
Kristoffer WEBER (Goethe University, Frankfurt, D) Lentiviral "gene ontology" (LeGO) vectors - a novel tool for functional gene analysis

12h40 - 14h00: Lunch & poster viewing

14h00 - 17h15: Session 2: Applications of lentiviral vectors (I)  Chair: Marina CAVAZZANA-CALVO

Johan RICHTER (University Hospital, Lund, S) LV and models for hematopoietic stem cell-targeted gene therapy
Juan Bueren (CIEMAT, Madrid, SP)   HIV vectors for gene therapy of inherited aplastic anemias (40 min)
Didier NEGRE (ENS-INSERM, Lyon, F)   SIV, FIV and other LV vectors (40 min)
16h00 - 16h30: coffee break
16h30 - 17h00: Selected communications (15 min)
Marianne BLOCKMANS (ULB, Brussels, B) Generation of transgenic mice overexpressing lipid phosphatases by lentiviral technology
Tomas BOS (VUB, Brussels, B) Lentiviral shRNA multiplexing using double copy vectors

17h00 - 18h00: Plenary Lecture:  Frederic Bushman (Pennsylvania Med School, Philadelphia, PA, USA) Retroviral integration biology

July 1^st

9h00 - 12h00: Session 3: Applications of lentiviral vectors (II)  Chair: Boro DROPULIC  
Nicole DEGLON (CEA, Orsay, F)   Lentiviral vectors for the study and treatment of neurological disorders (40 min)
Kyriacos Mitrophanous (Oxford Biomedica, London, UK) EIAV (40 min)
Selected communications (15 min)
Christine HOHENADL (University of Veterinary Medicine,Vienna, A) Evaluation of multilevel neuroprotective approach for gene therapy of Parkinson's disease
Ralf JOKERS (INSERM, Paris, F) Silencing of OB-RGRP in mouse hypotalamic arcuate nucleus regulates leptin receptor function and prevent diet-induced obesity 
10h50 - 11h10: coffee break
Christopher BAUM   (Hannover Medical School, D)    Retroviral oncogenesis and vector design (40 min)
Frédéric PAQUES (CELLECTIS, Romainville, F) Meganucleases for gene therapy of inherited diseases (15 min)

12h05 - 13h00: Lunch & poster viewing (short lunch)

13h00 - 14h50: Session 4: Infections and immunity      Chair: Anne GALY 

David KLATZMANN (Pitié-Salpêtrière Hospital, Paris, F)  Vaccination and tolerization with vectors (40 min)
Ignacio ANEGON (INSERM-ITERT, Nantes, F) Generation of transgenic rats using lentiviral vectors and their use in immunology and regenerative medicine (15 min)
Ben BERKHOUT (CINIMA, Amsterdam, NL) Lentiviral vector coding shRNA against HIV (40 min)
Selected communication (15 min)
Keryn WILLIAMS (Flinders University, Adelaide, AU) Efficacy of steroid-inducible promoter for lentivirus-mediated transduction of cornea
14h50 - 15h00: coffee break
15h00 - 17h30: Session 5: Production and manufacturing  Chair: Patrice DENEFLE   
Sam STEPHEN (University College, London, UK)    The development of stable packaging cells (30 min)
Otto MERTEN (Généthon, Evry, F)   Large-scale manufacture of lentiviral vectors in GMP (30 min)
Selected communications (15 min)
Francesca BELLINTANI (MOLMED SpA, Milan, I) Large scale process for the production and purification of lentiviral vectors for clinical applications
Hanna LESCH (University of Kuopio, Kuopio, FI) Generation of lentiviral vectors using recombinant baculoviruses
Martin SCHLEEF (Plasmid Factory, Bielefeld, D) Large scale  minicircles DNA production becomes a reality
Boro DROPULIC (Lentigen, Elkridge, MD, USA) (title to be communicated) (30 min)
17h30 - 17h45: coffee break
17h45 - 19h15: Round Table: Industrial and business development provisional participation of: Anne-Marie MASQUELIER (Généthon/GENOSAFE, Evry, F),  Françoise RUSSO-MARIE (GENOPOLE, Evry, F), Boro DROPULIC (LENTIGEN, Elkridge, MD,USA), Supriya SHIVAKUMAR (SIGMA-ALDRICH, San Louis, MO, USA), Francesca BELLINTANI (MOLMED, Milan, I), Francis DUPONT (HENOGEN, Gosselies, B), and  other companies  (programme to be finalised)

20h30: Social event

July 2^nd

9h00 - 12h00: Session 6: Insertion, genotoxicity and biosafety/Clinical studies  Chair: Philippe LEBOULCH  
Jean Christophe PAGES (CHRU Trousseau, Tours, F)  Regulatory aspects of GMO and LV use in lab and clinic (40 min)
Eugenio MONTINI (TIGET, Milan, I)   Assessing genotoxicity in vivo: murine tumor-prone models (40 min)
Bruce LEVINE (University of Pennsylvania, Philadelphia, PA, USA) The first LV gene therapy trial: Mobilizing vectors to fight HIV (40 min)
11h00 - 11h20: coffee break 
Nathalie Cartier (INSERM UMR 745, Paris, F) The first LV gene therapy trial in France: Adrenoleukodystrophy (40 min)
Gerard MCGARRITY (VIRxSIS, Gaitersbourg,MD, USA) Patient safety monitoring in lentiviral vector gene therapy trials (15 min)

12h15 - 13h05: Plenary Lecture:  Luigi NALDINI (TIGET, Milan, I)   Novel LV systems and targeted insertion

13h05 - 13h15: Closing remarks and remission of the prizes  Anne GALY and Mauro MEZZINA 

13h15 - 14h30: Lunch   END of the Symposium         

Available for 200 participants only.  Register now!    Many places are still  available                   

Practical course (July 2^nd – 5^th)

Proposed experiments: 
▪ Production of a concentrated lentiviral vector using several methods 
▪ Titration of a lentiviral vector using infectious genome measures 
▪ Transduction of target cells 
▪ Tips for cloning into lentiviral vector and for qPCR technologies for vector analysis 
▪ Bio-informatic analysis of vector insertion sites  
▪ Meet-the-expert sessions
A debriefing of the practical course will occur on Saturday July 5^th to allow the participants to review all important items and to ensure maximal communication between participants and teachers.

List of 30 selected students